Photoactivatable fluorophores for durable labelling of individual cells

Literature Information

Publication Date 2021-05-11
DOI 10.1039/D1CC01488A
Impact Factor 6.222
Authors

Hiroki Kashima, Mako Kamiya, Shotaro Nakano, Masayuki Miura



Abstract

We have designed and developed non-fluorescent, cell-permeable photoactivatable fluorophores, photoactivatable SPiDERs (paSPiDERs), which exhibit fluorescence activation upon light irradiation, accompanied by the generation of a quinone methide intermediate that binds covalently to intracellular proteins. The fluorescence signal is durable for 24 hours, resistant to fixation and compatible with immunostaining, and selective cell labeling can be achieved at single-cell resolution.

Source Journal

Chemical Communications

Chemical Communications
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