Photoactivatable fluorophores for durable labelling of individual cells

文献情報

出版日 2021-05-11
DOI 10.1039/D1CC01488A
インパクトファクター 6.222
著者

Hiroki Kashima, Mako Kamiya, Shotaro Nakano, Masayuki Miura



要旨

We have designed and developed non-fluorescent, cell-permeable photoactivatable fluorophores, photoactivatable SPiDERs (paSPiDERs), which exhibit fluorescence activation upon light irradiation, accompanied by the generation of a quinone methide intermediate that binds covalently to intracellular proteins. The fluorescence signal is durable for 24 hours, resistant to fixation and compatible with immunostaining, and selective cell labeling can be achieved at single-cell resolution.

掲載誌

Chemical Communications

Chemical Communications
CiteScore: 8.6
自己引用率: 4.7%
年間論文数: 2458

ChemComm publishes urgent research which is of outstanding significance and interest to experts in the field, while also appealing to the journal’s broad chemistry readership. Our communication format is ideally suited to short, urgent studies that are of such importance that they require accelerated publication. Our scope covers all topics in chemistry, and research at the interface of chemistry and other disciplines (such as materials science, nanoscience, physics, engineering and biology) where there is a significant novelty in the chemistry aspects. Major topic areas covered include: Analytical Chemistry Catalysis Chemical Biology and medicinal chemistry Computational Chemistry and Machine Learning Energy and sustainable chemistry Environmental Chemistry Green Chemistry Inorganic Chemistry Materials Chemistry Nanoscience Organic Chemistry Physical Chemistry Polymer Chemistry Supramolecular Chemistry

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