Biosynthesis of violacein: a genuine intermediate, protoviolaceinic acid, produced by VioABDE, and insight into VioC function
Literature Information
Kouhei Shinoda, Takuji Hasegawa, Hiroaki Sato, Masaaki Shinozaki, Hirotomo Kuramoto, Yosuke Takamiya, Tsutomu Sato, Naoki Nikaidou, Takeshi Watanabe, Tsutomu Hoshino
A biosynthetic intermediate of violacein produced by the mixed enzymes of VioABDE was elucidated to be 5-(5-hydroxy-1H-indol-3-yl)-3-(1H-indol-3-yl)-1H-pyrrole-2-carboxylic acid, named protoviolaceinic acid, indicating that VioC, responsible for the final biosynthetic step, works to oxygenate at the 2-position of the right side indole ring, and that the oxygenation reaction to form the central pyrrolidone core proceeds in a non-enzymatic fashion.
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Source Journal
Chemical Communications

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