Engineering in vitro models of cystic fibrosis lung disease using neutrophil extracellular trap inspired biomaterials
Literature Information
Allison Boboltz, Sydney Yang, Gregg A. Duncan
Cystic fibrosis (CF) is a muco-obstructive lung disease where inflammatory responses due to chronic infection result in the accumulation of neutrophil extracellular traps (NETs) in the airways. NETs are web-like complexes comprised mainly of decondensed chromatin that function to capture and kill bacteria. Prior studies have established excess release of NETs in CF airways increases viscoelasticity of mucus secretions and reduces mucociliary clearance. Despite the pivotal role of NETs in CF disease pathogenesis, current in vitro models of this disease do not account for their contribution. Motivated by this, we developed a new approach to study the pathobiological effects of NETs in CF by combining synthetic NET-like biomaterials, composed of DNA and histones, with an in vitro human airway epithelial cell culture model. To determine the impact of synthetic NETs on airway clearance function, we incorporated synthetic NETs into mucin hydrogels and cell culture derived airway mucus to assess their rheological and transport properties. We found that the addition of synthetic NETs significantly increases mucin hydrogel viscoelasticity. As a result, mucociliary transport in vitro was significantly reduced with the addition of mucus containing synthetic NETs. Given the prevalence of bacterial infection in the CF lung, we also evaluated the growth of Pseudomonas aeruginosa in mucus with or without synthetic NETs. We found mucus containing synthetic NETs promoted microcolony growth and prolonged bacterial survival. Together, this work establishes a new biomaterial enabled approach to study innate immunity mediated airway dysfunction in CF.
Related Literature
IF 6.222
Near infrared light activation of an injectable whole-cell cancer vaccine for cancer immunoprophylaxis and immunotherapyIF 6.843
Front coverIF 6.843
Life cycle assessment of plasma-assisted ethylene production from rich-in-methane gas streamsIF 6.367
Novel aqueous amine looping approach for the direct capture, conversion and storage of CO2 to produce magnesium carbonateIF 6.367
Photoactivatable fluorophores for durable labelling of individual cellsIF 6.222
In situ growth of all-inorganic perovskite nanocrystals on black phosphorus nanosheetsIF 6.222
Highly efficient and durable III–V semiconductor-catalyst photocathodes via a transparent protection layerIF 6.367
Engineering nanoporous organic frameworks to stabilize naked Au clusters: a charge modulation approachIF 6.222
A hollow neuronal carbon skeleton with ultrahigh pyridinic N content as a self-supporting potassium-ion battery anodeIF 6.367
Source Journal
Journal of Materials Chemistry B
Journal of Materials Chemistry A, B & C cover high quality studies across all fields of materials chemistry. The journals focus on those theoretical or experimental studies that report new understanding, applications, properties and synthesis of materials. The journals have a strong history of publishing quality reports of interest to interdisciplinary communities and providing an efficient and rigorous service through peer review and publication. The journals are led by an international team of Editors-in-Chief and Associate Editors who are all active researchers in their fields. Journal of Materials Chemistry A, B & C are separated by the intended application of the material studied. Broadly, applications in energy and sustainability are of interest to Journal of Materials Chemistry A, applications in biology and medicine are of interest to Journal of Materials Chemistry B, and applications in optical, magnetic and electronic devices are of interest to Journal of Materials Chemistry C. More than one Journal of Materials Chemistry journal may be suitable for certain fields and researchers are encouraged to submit their paper to the journal that they feel best fits for their particular article. Example topic areas within the scope of Journal of Materials Chemistry B are listed below. This list is neither exhaustive nor exclusive. Antifouling coatings Biocompatible materials Bioelectronics Bioimaging Biomimetics Biomineralisation Bionics Biosensors Diagnostics Drug delivery Gene delivery Immunobiology Nanomedicine Regenerative medicine & Tissue engineering Scaffolds Soft robotics Stem cells Therapeutic devices image block All articles published in Journal of Materials Chemistry B from 2019 onwards will be indexed in MEDLINE®. Articles that primarily focus on providing insight into the underlying science and performance of biomaterials within a biological environment are more suited to our companion journal, Biomaterials Science.
Recommended Compounds
![37845-14-0 - 4,5-Dihydronaphtho[1,2-c]furan-1,3-dione](/structs/378/37845-14-0-f8d0.webp "37845-14-0 - 4,5-Dihydronaphtho[1,2-c]furan-1,3-dione")
Recommended Suppliers
Baoding Lutheran Fine Chemical Co., Ltd.
Albert Handtmann Elteka GmbH & Co. KG
LOBA Feinchemie AGBerghof GmbHShenyang Xikai Automation Equipment Co., Ltd.Retec Elektronische Regeltechnik GmbHLianwater County Yishun Environmental Protection Co., Ltd.Taizhou Jianjiang Tianyi Chemical FactoryShanghai Biosundrug Co., LtdCarl Zeiss Automated Inspection GmbH & Co. KG