Pea protein extraction method impacts the protein (micro)structural organisation and in vitro digestion kinetics

Literature Information

Publication Date 2024-01-04
DOI 10.1039/D3FO04225A
Impact Factor 5.396
Authors

Sarah H. E. Verkempinck, Dorine Duijsens, Ankita Mukherjee, Peter J. Wilde



Abstract

There is increasing interest in including pulse proteins into food products due to their nutrient-rich and sustainable character. However, little is known regarding the consequences of different extraction approaches on the pulse protein structure and the subsequent protein (micro)structural organization and protein digestion kinetics. Therefore, three green pea protein extracts were created: (i) cooking followed by cotyledon cell isolation, (ii) alkaline extraction followed by isoelectric precipitation, or (iii) salt extraction, and compared to the original pea flour as well as to sodium caseinate. The results showed that encapsulated, denatured protein inside pea cotyledon cells presented the (s)lowest digestion, while accessible and more native protein (e.g., pea flour, pea protein salt extract) presented much faster and higher digestion. Moreover, the alkali extracted pea protein was denatured to some extent, significantly lowering in vitro digestion kinetics. In the second part, three different in vitro approaches were applied to digest the salt extracted pea protein. Semi-dynamic gastric digestion approaches simulate in vivo conditions more closely which especially impacted the rate of digestion.

Source Journal

Food & Function

Food & Function
CiteScore: 10.1
Self-citation Rate: 3.9%
Articles per Year: 758

Food & Function provides a unique venue for physicists, chemists, biochemists, nutritionists and other food scientists to publish novel, cutting-edge, original research focussing on food, its nutrients and their relation to human health and nutrition. We welcome research describing the: Physical properties and structure of food and how this relates to sensory perception and human health Biochemical and physiological actions of food components Interactions between foods, gut microbiota and human physiology Nutritional and biological evaluation of food Clinical and population studies using food or food components Development of biomarkers of food intake and effects on human health We also welcome systematic reviews and meta-analyses of existing studies in the literature, provided these are objective and scientifically valid Food in this context is defined as materials of plant, animal or mineral origin, which are consumed orally (by humans) for pleasure and to sustain growth and vital processes. Examples of research topics that are of interest to be published in Food & Function are: Chemistry and physics of food components and digestion processes Relationship between the physical properties/structure of food and nutrition and human health - for example, impact of food matrix or processing on nutrient release and uptake Molecular properties and physiological effects of food components (nutrients, fibres, essential micronutrients, phytochemicals, bioactives, food substitutes, novel ingredients, allergens, flavours and fragrances) Nutritional and health effects of food including bioavailability and metabolism assessment of food components (nutrients, micronutrients and other microconstituents) Efficacy and mechanisms of food constituents in the body - including biomarkers of intakes, exposure and effects Impacts of foods/food components on gut microorganisms and human physiology - For example impact of fermented foods Role of nutrition and diet in human disease prevention and development Cellular and molecular effects/mechanisms of food/food components image block The following types of research are not within the scope of Food & Function: Research relating to traditional herbal medicines, medicinal plants or active compounds extracted from such plants (materials that are primarily consumed as medicine, i.e. the intended purpose is primarily to treat, cure or prevent a non-deficiency disease) or relating to foods not recognised as human diet contributors Animal nutrition research that is not primarily designed as a model to benefit human nutrition (for example, studies of growth/accretion, heat stress, weaning, ruminant digestion, meat quality, etc.) Treatments administered by non-oral routes such as injection (subcutaneous, intramuscular, intraperitoneal, etc.), dermal/transdermal, rectal, inhalation, nasal, etc. Exceptions are when such routes of administration are used for mechanistic/control purposes in the experimental design Pharmacological/pharmaceutical approaches: Encapsulation, emulsification and/or pure controlled release of compounds or bioactives that do not come directly from edible foods, such as dietary supplements - these are better suited to a pharmaceutical journal In vitro or in vivo studies with poorly defined (insufficiently characterised) extracts and studies without appropriate controls will not be considered Cells studies not considering the metabolism of food components ingested – for example, irrelevant exposure of cells to compounds not present in the body after absorption Manuscripts with only a fully theoretical/bioinformatic approach and without appropriate support from analytical evidence will not be considered for publication Studies focussing solely on food engineering, preservation and sustainable technologies – these can be published in our companion journal Sustainable Food Technology Pure food analysis - these can be published in Analytical Methods

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