Design of liposome-based pH sensitive nanoSPINprobes: nano-sized particles with incorporated nitroxides
文献信息
Andrey A. Bobko, Igor A. Kirilyuk, Julya F. Polienko, Maxim A. Voinov, Elena G. Bagryanskaya, Valery V. Khramtsov
Liposome-based nanoSized Particles with Incorporated Nitroxides, or nanoSPINs, were designed for EPR applications as pH probes in biological systems. Phospholipid membrane of the liposomes with incorporated gramicidin A showed selective permeability to a small analyte, H+, while protecting entrapped sensing nitroxide from biological reductants. An application of the pH-sensitive nanoSPIN in an ischemia model in rat heart homogenate allows for monitoring ischemia-induced acidosis while protecting encapsulated nitroxide against bioreduction.
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