Comparative study of poly(styrene-sulfonate)/poly(L-lysine) and fibronectin as biofouling-preventing layers in dissolved oxygen electrochemical measurements
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Raphaël Trouillon, Christine Cheung, Bhavik Anil Patel, Danny O'Hare
Oxygen is a major actor of many physiological, biological and industrial processes; as such, its monitoring is of paramount importance. The effects of protein biofouling on dissolved oxygen measurements are described. The consequences of protein adsorption on electrode kinetics and mass transport were quantified using cyclic voltammetry, AC impedance and rotating disc amperometry, firstly in a solution of albumin, secondly in a complex protein suspension of liver tissue. The effect of membrane coatings poly(styrene-sulfonate)/poly(L-lysine) and fibronectin were investigated. The relative significance on mass transport, surface diffusion and electrocatalysis were examined by comparing the experimental data for an outer-sphere redox couple, ruthenium hexaammine (III)/(II), with the physiologically significant electrocatalytic O2reduction reaction.
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Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.
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